EC Number | General Stability | Organism |
---|---|---|
2.7.4.14 | dialysis against 20 mM phosphate, 1 mM MgCl2, 20% ethylene glycol, pH 8.0, 90% loss of activity | Rattus norvegicus |
2.7.4.14 | freeze-thawing inactivates | Rattus norvegicus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.4.14 | DTNB | 0.009 mM, 50% inhibition | Rattus norvegicus | |
2.7.4.14 | iodoacetate | 0.05 mM, 50% inhibition | Rattus norvegicus | |
2.7.4.14 | NEM | 0.035 mM, 50% inhibition | Rattus norvegicus | |
2.7.4.14 | p-hydroxymercuribenzoate | 0.02 mM, 50% inhibition | Rattus norvegicus | |
2.7.4.14 | p-Hydroxymercuriphenyl sulfonate | 0.02 mM, 50% inhibition | Rattus norvegicus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.4.14 | 22500 | - |
sucrose density gradient centrifugation | Rattus norvegicus |
2.7.4.14 | 26000 | - |
gel filtration | Rattus norvegicus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.4.14 | Rattus norvegicus | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.7.4.14 | bone marrow | - |
Rattus norvegicus | - |
EC Number | Storage Stability | Organism |
---|---|---|
2.7.4.14 | -80°C, up to 12 months | Rattus norvegicus |
2.7.4.14 | 4°C, considerable loss of activity within 24 h, DTT stabilizes, more stable in 20 mM phosphate buffer, pH 8 than in Tris-HCl buffer | Rattus norvegicus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.4.14 | 4 | - |
24 h, in absence of dithiothreitol the purified enzyme shows considerable loss of activity | Rattus norvegicus |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
2.7.4.14 | Rattus norvegicus | isoelectric focusing | 4.8 | 4.7 |